One of the most challenging issues following restoration is microleakage. The aim of this study was to investigate the effects of Er,Cr:YSGG laser with and without acid etching on microleakage of class V composite restorations. A total of 68 human intact premolars were selected, disinfected, and randomly allocated to four experimental groups (n = 16) as well as positive and negative controls (n = 2 each). Dimensionally, similar class V cavities were prepared on buccal surface of each tooth under the following conditions: group 1, bur cavity preparation and chemical etching (BE); group 2, bur cavity preparation and Er,Cr:YSGG laser conditioning (BLc); group 3, Er,Cr:YSGG laser cavity preparation and chemical etching (LE); and group 4, Er,Cr:YSGG laser cavity preparation and Er,Cr:YSGG laser conditioning (LLc). All samples were restored with composite. The teeth were sealed and immersed in 1 % methylene blue for 48 h before being sectioned. The microleakage evaluation was done under a stereomicroscope (x20). The leakage scores were recorded and Kruskal-Wallis and Mann-Whitney tests were used for statistical evaluations. The highest microleakage score was seen in gingival margins of group 4 (LLc) and the lowest in occlusal margins of group 3 (LE). The overall difference in leakage scores among the groups was statistically significant (p < 0.001) with gingival margins showing a significantly higher score than its occlusal counterparts (p < 0.001). It was noted that less microleakage could be achieved when cavities were prepared by laser compared to bur. In addition, less microleakage was seen in acid-etched cavities than laser-conditioned counterparts.
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