Effects of resveratrol and irradiation upon oral squamous cell carcinoma cells.

OBJECTIVE: To evaluate the effects of resveratrol and irradiation on oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Resveratrol was administered at doses of 5, 10, 25, 50 and 100 microM to PE/CA-PJ15 (OSCC) cultures irradiated with different doses (1, 2.5 and 5 Gy). Effects upon cell viability, apoptosis and migration were evaluated after 24, 48 and 72 h incubation. RESULTS: After 72 h of incubation, the 100 microM dose of resveratrol induced the greatest decrease in cell viability at all irradiation doses. After 24, 48 and 72 h of incubation, 100 microM of resveratrol induced the greatest cell apoptosis at all irradiation doses. The greatest alterations in the distribution of the G0-G1, G2-M and S phases of the cell cycle were recorded with 50 and 100 microM of resveratrol; after 24, 48 and 72 h of incubation, both these doses resulted in an increase in the S phase, at the expense of the G0-G1 and G2-M phases. CONCLUSIONS: Resveratrol increases cytotoxic activity in the PE/CA-PJ15 cell line and reduces cell migration capacity, while the combination of resveratrol and irradiation exerts a synergic effect.