Randomised in situ trial on the effect of milk and CPP-ACP on dental erosion.

OBJECTIVES: This randomised in situ study aimed to analyse the effect of milk (with or without 5ppm F) and CPP-ACP pastes (with or without 900ppm F) on dental erosion. METHODS: The study was a seven phase (5 days each) crossover design involving 15 participants wearing intraoral appliances with enamel and dentine specimens. Specimens were extraorally eroded (erosive soft drink, 6x90s/day) and brushed (2x30s/day, 2N) using a non-fluoridated toothpaste (negative control). The test products were milk, milk+5ppm F (twice daily, each 100ml/2min), CPP-ACP paste, CPP-ACP paste+900ppm F (3min/day) or a SnCl2/AmF/NaF mouthrinse (positive control, 30s/day), which were applied immediately after erosion with the appliances in the oral cavity. In an additional group, a fluoridated toothpaste was used without any additional test product. Tissue loss was determined profilometrically after 5 days and statistically analysed by linear mixed models methodologies (p<0.05). RESULTS: Compared with the negative control (non-fluoridated toothpaste only, enamel: 2.2+/-1.3mum; dentine: 3.8+/-2.2mum), enamel and dentine loss was significantly reduced by the use of fluoridated toothpaste (enamel: 1.1+/-1.0mum; dentine: 2.4+/-1.7mum) and the SnCl2/AmF/NaF mouthrinse (1.5+/-1.5mum; dentine: 1.8+/-1.9mum). CONCLUSIONS: Milk and CPP-ACP were not effective in reducing enamel and dentine loss significantly, independently of the presence of fluoride. CLINICAL SIGNIFICANCE: Enamel and dentine erosion were significantly reduced by the use of a fluoridated toothpaste or a SnCl2/AmF/NaF mouthrinse, but not by milk or CPP-ACP under the conditions of the present study. CLINICAL TRIALS REGISTRATION: NCT01566357.