AMPK-dependent signaling modulates the suppression of invasion and migration by fenofibrate in CAL 27 oral cancer cells through NF-kappaB pathway.

Fenofibrate, a peroxisome proliferator-activated receptor alpha (PPARalpha) agonist and lipid-lowering agent, has been used worldwide for treatment of hyperlipidemia. The clinical trials demonstrate that fenofibrate possesses multiple pharmacological activities, including antitumor effects. However, the precise mechanisms in oral squamous cell carcinoma (OSCC) remain unclear. In this study, we investigated the anticancer effects of fenofibrate on the migration and invasion of human oral cancer CAL 27 cells. Fenofibrate inhibited the cell migration and invasion of CAL 27 cells by the wound healing and Boyden chamber transwell assays, respectively. In addition, fenofibrate reduced the protein expressions of MMP-1, MMP-2, MMP-7, and MMP-9 by Western blotting and inhibited enzyme activities of MMP-2/-9 using gelatin zymography assay. Results from immunoblotting analysis showed that the proteins of p-LKB1 (Ser428), LKB1, p-AMPKalpha (Thr172), p-AMPKalpha1/alpha2 (Ser425/Ser491), p-AMPKbeta1 (Ser108), and AMPKgamma1 were upregulated by fenofibrate; the levels of p-IKKalpha/beta (Ser176) and p-IkappaBalpha were reduced in fenofibrate-treated cells. Also, fenofibrate suppressed the expressions of nuclear NF-kappaB p65 and p50 by immunoblotting and NF-kappaB DNA binding activity by EMSA assay. The anti-invasive effect of fenofibrate was attenuated by compound C [an adenosine 5'-monophosphate-activated protein kinase (AMPK) inhibitor] or dominant negative form of AMPK (DN-AMPKalpha1). Thus, fenofibrate considerably inhibited metastatic behaviors of CAL 27 cells might be mediated through blocking NF-kappaB signaling, resulting in the inhibition of MMPs; these effects were AMPK-dependent rather than PPARalpha signaling. Our findings provide a molecular rationale, whereby fenofibrate exerts anticancer effects and additional beneficial effects for the treatment of cancer patients. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 866-876, 2016.