Immunohistochemical Expression of CD105 and TGF-beta1 in Oral Squamous Cell Carcinoma and Adjacent Apparently Normal Oral Mucosa and its Correlation With Clinicopathologic Features.

Angiogenesis in oral squamous cell carcinomas (OSCC) is essential for its growth, invasion, and metastasis. This entails a shift in the balance between proangiogenic and antiangiogenic factors. CD105 and TGF-beta1 are 2 such proangiogenic factors wherein CD105 exerts its angiogenic effect by binding to and modulating the TGF-beta1 pathway. A total of 50 resected specimens of OSCC were considered. One tissue specimen was taken from tumor proper and another specimen from adjacent apparently normal mucosa (AANM). Both tissues were immunohistochemically stained using CD105 and TGF-beta1 antibodies. The expression of each antibody was individually assessed and then compared. Pearson chi test was used for statistical comparison of expression. CD105 was significantly expressed in OSCC as compared with AANM and also correlated with increasing TNM stage. The mean microvessel density was higher in OSCC. TGF-beta1 was significantly expressed in epithelium of OSCC as compared with AANM. On comparing expression of TGF-beta1 and CD105, 79.54% of endothelial cells expressed positivity for both molecules. Both CD105 and TGF-beta1 were increased in OSCC, although based on our results CD105 alone can be used as a prognostic marker. On the basis of immunohistochemical expression of CD105 and TGF-beta1 in endothelial cells, our results demonstrate that CD105 acts as one of the receptors of TGF-beta1 on endothelial cells and induces the angiogenic pathway in OSCC.