Proinflammatory cytokine production from NOK-SI keratinocytes after exposure to denture adhesives.

STATEMENT OF PROBLEM: Although the cytotoxicity of denture adhesives has been widely assessed, data on the release of proinflammatory cytokines from oral epithelial cells are still scarce. PURPOSE: The purpose of this in vitro study was to evaluate the proinflammatory cytokines interleukin (IL) 1beta, IL-6, and tumor necrosis factor alpha (TNF-alpha) released from human oral keratinocytes after exposure to denture adhesives. MATERIAL AND METHODS: Eluates of the Ultra Corega cream, Corega powder Fixador Ultra, and Corega strip denture adhesives were created at 1% wt/vol after 24 hours of exposure in a Dulbecco modified Eagle medium culture. The release of IL-1beta, IL-6, and TNF-alpha was measured using a sandwich enzyme-linked immunosorbent assay after the oral epithelial cells (NOK-SI) had been exposed to the eluates for 3, 6, and 24 hours (n=6). RESULTS: IL-1beta release from the strip was higher at 3 hours compared with the control (Bonferroni post hoc test, P=.016). IL-6 releases from the cream and powder denture adhesives at 6 and 24 hours were higher than for the controls (Bonferroni post hoc test, P<.05). TNF-alpha release was not detectable in some groups and periods. CONCLUSIONS: In long periods, denture adhesive strips do not induce any change on cytokine release from human oral keratinocytes.