[Investigation of the effect of nuclear factor kappaB on inflammatory cell recruitment phenotype of oral cancer associated macrophage].

PURPOSE: To explore the ability of nuclear factor kappaB (NFkappaB) in sustaining inflammatory cell recruitment phenotype of oral cancer associated macrophages, by using NFkappaB inhibitor(-Bay11-7082). METHODS: By primary culture, murine macrophages were harvested. Cal27 conditioned medium (CM) and Bay11-7082 were applied for stimulation of the macrophages. RT-PCR and ELISA were used for detecting the inflammatory cell recruitment related chemotactic factors. GraphPadPrism5 was used for statistical analysis. RESULTS: Bay11-7082 prevented the contour change into a spindle shape via Cal 27 CM. It also attenuated MCP-1, GM-CSF, MCP-5 and CCL-5 mRNA increase after Cal 27 CM stimulation (P<0.05). At protein level, impeding NFkappaB activation could significantly prevent MCP-1 and GM-CSF secretion from oral cancer associated macrophage (P<0.001). CONCLUSIONS: NFkappaB signaling may play a key role in sustaining the inflammatory cell recruitment phenotype of oral cancer associated macrophages.