Objective: The aim of this study was to investigate the hemodynamic effects of occlusal splint therapy on masseter muscles of patients with myofascial pain accompanied by bruxism with near-infrared spectroscopy (NIRS). Methods: Twenty-four patients were randomly divided into two groups, where the study group (n = 12) received occlusal splint therapy and the control group (n = 12) underwent no therapy. Measurements were categorized into four subgroups: painful or painless control and painful or painless splint. Percent changes in deoxyhemoglobin (Hb), oxyhemoglobin (HbO(2)), and OXY (HbO(2)-Hb) values were calculated during a 1-month period. Results: Statistically significant inter-session differences between painful-splint and painful-control groups were detected for NIRS oxygenation parameters, whereas inter-session differences between painless groups were statistically insignificant. Conclusion: The results suggest that occlusal splint usage causes a decrease in hyperemic response, which is indicative of a decrease in masseter muscle contraction strength.
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