Effect of technetium-99 conjugated with methylene diphosphonate ((99) Tc-MDP) on OPG/RANKL/RANK system in vitro.

BACKGROUND: RANKL and RANK play an important role in jaw resorption during the development of the ameloblastomas. Therefore, the aim of this study was to explore the effect of (99) Tc-MDP on OPG/RANKL/RANK system on RAW264.7 and MC3T3-E1 cell lines in vitro and provide the theoretical basis for the clinical treatment of the jaw ameloblastoma. METHODS: Different concentrations of (99) Tc-MDP were used to treat RAW264.7 and MC3T3-E1 cell lines. The cell proliferative inhibition rate was analyzed by CCK-8. Cell apoptosis and cell cycle were detected by flow cytometry. Western blot was used to detect the expression of OPG, RANKL, and RANK. RESULTS: Treatment of RAW264.7 cell lines with different concentrations of (99) Tc-MDP had inhibitory effects and decreased the expression of RANK protein. The cell proliferation of (99) Tc-MDP on MC3T3-E1 cell lines was stronger at 48 hours than at 24 hours except for 100 mug/mL concentration group. Compared with the concentration of 0.01 mug/mL, the treatment of MC3T3-E1 cells with 100 mug/mL (99) Tc-MDP showed that the cell proliferative effect decreased at 24 hours and 48 hours (P < 0.05). After treatment with 0.01 mug/mL (99) Tc-MDP, the expression of OPG in MC3T3-E1 cells was significantly increased (P < 0.05). Compared with 0.01 mug/mL, the expression of RANKL was decreased after treatment with 100 mug/mL (99) Tc-MDP (P < 0.05). CONCLUSION: (99) Tc-MDP can induce apoptosis of RAW264.7 cells and inhibit the expression of RANK protein. The effect of 0.01 mug/mL of low concentration of (99) Tc-MDP can promote the proliferation of MC3T3-E1 cells and increase the expression of OPG and RANKL protein. (99) Tc-MDP may have adjuvant therapeutic effects on the treatment of jaw ameloblastoma.