MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-kappaB system-mediated epithelial-mesenchymal transition.

Tongue squamous cell carcinoma (TSCC) is one of the most common incident oral cancers, which is accompanied by high rate of metastasis and recurrence. It has been demonstrated that elevated interleukin-8 (IL-8) promoted metastasis of various cancers via regulating epithelial-mesenchymal transition (EMT) process, whereas the accurate mechanism is left to be elucidated. The present work was aimed to investigate the role of microRNA-940 (miR-940)/C-X-C chemokine receptor type 2 (CXCR2) system in the metastasis ability and EMT process of IL-8-treated TSCC cells and further explore the underlying mechanisms. We found that miR-940 up-regulation inhibited IL-8-induced migration and invasion, which could be deprived by CXCR2 silence. We also observed that miR-940 suppressed epithelial marker E-cadherin expression while increased mesenchymal markers N-cadherin and Twist levels in IL-8-stimulated TSCC cells. Besides, IL-8-induced invasion and EMT process of TSCC cells were impeded in the present of the NF-kappaB inhibitor, PDTC or BAY117082. In conclusion, our data demonstrated that miR-940/CXCR2 system regulated the metastasis of TSCC cells via NF-kappaB-induced EMT process.