This in vitro study investigated whether aging of restorative materials influences secondary caries development. The research question was whether antibacterial bonding maintained its effectiveness after aging. 60 enamel-dentine blocks were restored using 3 different restorative materials (n = 20 per material): composite with conventional bonding, composite with antibacterial bonding and amalgam. Half of the samples in each group (n = 10) were artificially aged through exposure to a sucrose biofilm model. Lesion depth was subsequently measured using transversal microradiography. Aging led to deeper lesions in the composite with antibacterial bonding group (CAB), compared to fresh samples. These fresh samples showed lesions less deep than fresh samples of composite with conventional bonding. After aging, this effect was absent. Within the limitations of this short-term in vitro study, it can be concluded that aging of restorative materials can influence caries progression. Antibacterial bonding materials lose their effectiveness over time.
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