OBJECTIVE: To evaluate the influence of the file format of digital periapical radiographs on the diagnosis of vertical root fracture (VRF). STUDY DESIGN: Periapical radiographic images of 34 single-rooted teeth-19 with VRF, and 15 without VRF were acquired using two digital systems-Digora Toto, and Digora Optime, and exported into four different file formats-TIFF, BMP, PNG, and JPEG, totaling 272 radiographs. The radiographs were assessed by five examiners for the detection of VRF, using a 5-point scale (1-definitely absent; 2-probably absent; 3-uncertain; 4-probably present; 5-definitely present). Diagnostic values of area under the ROC curve, specificity, and sensitivity for the diagnosis of VRF were calculated. The results were compared by two-way Analysis of Variance with post hoc Tukey's test. The intra- and inter-examiner agreements were measured by the Kappa test. The significance level was set at 5% for all analyses. RESULTS: The values of intra-examiner agreement varied from moderate (0.56) to almost perfect (0.81), while the values of inter-examiner agreement varied from fair (0.29) to moderate (0.60). The image file format did not influence the diagnostic values for VRF for any of the radiographic systems tested (p > 0.05). Digora Toto had significantly greater values of area under the ROC curve than Digora Optime for all file formats (p = 0.001). CONCLUSION: The image file format of periapical radiographs does not influence the diagnosis of VRF, regardless of the digital radiography system.
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