The aim of this study was to compare the cyclic fatigue resistance of nickel-titanium instruments inside single and double-curved canals at different temperatures. 160 HyFlex EDM #20.05 (HEDM), VDW.ROTATE #20.05 and #25.06, Mtwo #25.06 were randomised (n = 10) for the dynamic cyclic fatigue tests according to the curvature (i.e. single and double) at 20 degrees +/- 1 degrees C and 35 degrees +/- 1 degrees C. The number of cycles to fracture (NCF) was analysed by two-way ANOVA with p < 0.05. Fatigue resistance of all instruments significantly decreased at body temperature in single and double curvatures, except for HEDM in double curvature. The NCF was significantly lower in double curvature than single at both temperatures for all files, except for VDW.ROTATE #20.05 at 35 degrees +/- 1 degrees C. Within the study limitations, temperature significantly impaired cyclic fatigue resistance of all files except HEDM #20.05 in double curvature. Similarly, double curvature had a detrimental effect on cyclic fatigue resistance of all files except for VDW.ROTATE #20.05 at body temperature.
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