[Study of properties of three-dimensional matrices manufactured by antisolvent 3D printing for reconstruction of extensive bone defects in dentistry and maxillofacial surgery].

OBJECTIVE: The aim of the study to study of physical, mechanical and biocompatible properties of the matrices manufactured by antisolvent 3D printing from the solutions of polylactide-co-glycolide in tetraglycol. MATERIAL AND METHODS: Three-dimensional scaffolds were made from a solution of polylactide-co-glycolide mixed with tetraglycol using antisolvent 3D printing. The elastic properties and the structure of the obtained matrices were studied. MTT-test and staining with PKH-26, Calcein-AM, DAPI with subsequent fluorescence microscopy were used to study biological properties. RESULTS: The three-dimensional scaffolds had good mechanical properties. Young's modulus value was 18+/-2 MPa, tensile strength was 0.43+/-0.05 MPa. The relative survival rate of cells after the first day was 99.58+/-2.28%, on the 14th day - 98.14+/-2.22%. The structure of the scaffold promoted cell adhesion and spreading on its surface. CONCLUSION: The polylactide-co-glycolide matrices produced by antisolvent printing have high porosity, biocompatibility and good mechanical properties. It is allowed to use them in the future as a basis for personalized constructions for the replacement of extensive bone defects of the maxillofacial region.