INTRODUCTION: In this study, the ability to remove obturation material in canals filled with Bio C Sealer, BC Sealer Hiflow, and AH Plus was compared using micro-computed tomography. MATERIALS AND METHODS: In phase 1, 45 premolars were prepared with Reciproc Blue 25 files and divided into 3 experimental groups according to sealer and obturation techniques (n = 15): Group HI - BC Sealer Hiflow and continuous wave technique; Group AH - AH Plus and continuous wave technique; Group BC - Bio C Sealer and single cone technique. The teeth were stored for 30 days and scanned by micro-computed tomography to evaluate the volume of the obturation material. In phase 2, the teeth were unblocked with the Reciproc R40 file and the Flatsonic ultrasonic insert and scanned again. In phase 3, all samples were subjected to passive ultrasonic irrigation and a final scan was performed. The volume of obturation material was compared across all phases and groups. The volume of obturation material was calculated for the entire canal using an ANOVA analysis with a significance level of 5%. RESULTS: The BC group showed a significant difference compared to the AH group (P = .005) and HI group (P < .001). There was no significant difference between the AH and HI groups. Within the groups, only the comparison of phase 2-3 in the HI group showed no significant difference (P = .005). No group achieved complete removal of the obturation material. CONCLUSION: Bio C Sealer had the least residual obturation material and passive ultrasonic irrigation improved material removal in AH Plus and Bio C Sealer sealers, but not in the BC Sealer Hiflow sealer.
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